IL-10 Secreting Dendritic Cell Line (MuTu DC1940-IL-10)

Propagation

Please contact us for propagation details.

Additional Information

The MuTu DC1940-IL-10 cell line is derived from murine tumor (MuTu) dendritic cells (DC) expressing IL-10. To generate the IL-10 DC line, IL10 gene was obtained from the cDNA of MuTuDC1s stimulated with CpG and amplified by PCR. DNA fragments were purified and inserted into lentiviral vectors. Transgene expression and protein production was confimed through FACS and ELISA. Cells produce IL-6 and IL12p40 when stimulated with CpG1826.This cell line is a novel stable, and functional DC line model for DC related research.