N2a Split Superfolder GFP-Tau (N2a-ssGT) Cell Line

Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% heat-inactivated FBS + 1% Glutamax + 1% NEAA (TM068) + 1% Sodium pyruvate (TM057) + 125 µg/ml Zeocin (Gibco, #R25001, 100 mg/ml) + 400 µg/ml G418 (G271) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂

Cells were stably transfected with pmGFP10C-Tau and pmGFP11C-Tau plasmids