FLT3-ITD Mutant 32D Stable Cell Line (32D-FLT3-ITD)

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.PriGrow II (TM002) + 10%HI-FBS+10 ng/mL recombinant murine IL-3 (required) +1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells grow slowly initially.

This cell line features a stable mutation in the internal tandem duplication (ITD) - the most common mutation in Fms-like tyrosine kinase (FLT3), often implicated in acute myeloid leukemia (AML). As FLT3-ITD mutations are associated with AML through its role in cell proliferation and tumor formation, this cell line is suitable for studies investgating the association of FLT3-ITD in AML formation and progression. Additional cell lines from this panel:Cat. T3512 - Wildtype FLT3 32D Stable Cell Line (32D-FLT3-WT)Cat. T3513 - ALK-F1174L Stable Mutant Ba/F3 Cell Line (Ba/F3 ALK-F1174L)Cat. T3514 - FLT3-D835Y Mutant Ba/F3 Stable Cell Line (Ba/F3-FLT3-D835Y)Cat. T3515 - FLT3-ITD Mutant 32D Stable Cell Line (32D-FLT3-ITD)Cat. T3516 - FLT3-D835Y Mutant 32D Stable Cell Line (32D-FLT3-D835Y)