U251 Cells
Specifications | |
---|---|
Cell Category: | Cancer Cells & Cell Lines |
Organism: | Human (H. sapiens) |
Tissue of Origin: | Brain |
Morphology: | Pleomorphic/astrocytoid |
Culture Properties: | Adherent |
Donor Age: | 75 |
Donor Gender: | Male |
Pathology: | Astrocytoma |
Propagation
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1 nM Sodium Pyruvate (TM057) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Alternative media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate
Additional Information
The U251 (or U-251) cell line is derived from a malignant astrocytoma tumor in a 75-year-old male and serves as a model for glioblastoma multiforme (GBM), the most common and aggressive form of brain cancer.
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