U251 Cells

Specifications
Cell Category:Cancer Cells & Cell Lines
Organism:Human (H. sapiens)
Tissue of Origin:Brain
Morphology:Pleomorphic/astrocytoid
Culture Properties:Adherent
Donor Age:75
Donor Gender:Male
Pathology:Astrocytoma

Propagation

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1 nM Sodium Pyruvate (TM057) + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Alternative media: Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate

Additional Information

The U251 (or U-251) cell line is derived from a malignant astrocytoma tumor in a 75-year-old male and serves as a model for glioblastoma multiforme (GBM), the most common and aggressive form of brain cancer.

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Maria Schröder

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