Immortalized Human Colonic Epithelial Cells (HCEC-1CT)
| Specifications | |
|---|---|
| Cell Category: | Immortalized Cell Lines |
| Organism: | Human |
| Tissue of Origin: | Colon |
| Morphology: | Compact cuboidal to spindle-shaped cells |
| Culture Properties: | Adherent |
| Immortalization Method: | Immortalized with retroviral Cdk4 and Human telomerase reverse transcriptase (hTERT) |
| Marker: | LGR5, BMI1, CD29, and CD44 In proliferation: vimentin, α-smooth muscle actin Upon growth arrest: cytokeratin 18, zonula occludens-1, mucins-1 and -2, antigen A33, and dipeptidyl peptidase 4 |
Propagation
Please contact us for propagation details.
Additional Information
Immortalized Human Colonic Epithelial Cells (HCEC-1CT) is a human colonic epithelial cell (HCEC) line isolated from healthy adult patient biopsy and immortalized by transduction with the cyclin-dependent kinase 4 (Cdk4) and human telomerase reverse transcriptase (hTERT). HCEC-1CT cells express stem cell markers, such as LGR5, BMI1, CD29, and CD44, and retain multilineage epithelial differentiation capability. Thus, HCEC-1CT cells are capable of differentiating into various epithelial lineages of the colon and forming self-organizing cysts in 3D organotypic culture in Matrigel. During differentiation, HCEC-1CT cells express colon epithelial cell-specific markers such as A33 and mucin 1. Compared to most HCEC cell lines of malignant origin, HCEC-1CT is a valuable cytogenetically normal and nontumorigenic cell line for studying colon stem cell biology, differentiation as well as the initiation and progression of colonic diseases, such as colorectal cancer.
Commercial Price Disclaimer
Researchers at for-profit organizations and corporations, please contact us for pricing of this item.
Genetically Modified Organism (GVO)
See General Terms.
- Catalog Number
T0715-GVO-ABM - Supplier
ABM - Size
- Shipping
Dry Ice
- Price
- Please inquire
