OxiSelect™ Advanced Glycation End Product (AGE) Competitive ELISA Kit

Product Description

• Detect advanced glycation end product formation as low as 0.5 ug/mL
• AGE-modified BSA and Reduced BSA included as standards
• Compatible with cell lysates, plasma, serum, and purified proteins

Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins.

The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent advanced glycation end products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage.

Cell Biolabs' OxiSelect™ AGE Competitive ELISA kit is designed for rapid detection and quantitation of advanced glycation end product protein adducts. The quantity of AGE adduct in protein samples is determined by comparing its OD with that of a known AGE-BSA standard curve.

Assay Principle:

First, an AGE conjugate is coated on an ELISA plate. The unknown AGE protein samples or AGE-BSA standards are then added to the AGE conjugate preabsorbed ELISA plate. After a brief incubation, an anti-AGE polyclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of AGE protein adducts in unknown samples is determined by comparison with a predetermined AGE-BSA standard curve.