OxiSelect™ In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)
| Specifications | |
|---|---|
| Research Area: | Oxidative Stress |
| Assay Category: | Nitric Oxide (NO) Assays |
| Sample Type: | Suitable for serum, plasma, urine, saliva, lysates, and media |
| Detection Method: | Colorimetric |
Product Information
Cell Biolabs’ OxiSelect™ Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume).
Product Citations
- Sheng Cheng, H. (2017) The Ameliorative Effects of a Tocotrienol-Rich Fraction on the AGE-RAGE Axis and Hypertension in High-Fat-Diet-Fed Rats with Metabolic Syndrome. Nutrients. doi: 10.3390/nu9090984
- Son, D.J. et al. (2016) Novel synthetic (E)-2-methoxy-4-(3-(4-methoxyphenyl) prop-1-en-1-yl) phenol inhibits arthritis by targeting signal transducer and activator of transcription 3. Sci Rep. 6:36852.
- Vincent, A. et al. (2016) Herring and chicken/pork meals lead to differences in plasma levels of TCA intermediates and arginine metabolites in overweight and obese men and women. Mol Nut. doi:10.1002/mnfr.201600400.
- Wang, Z. et al. (2015) Dual-microstructured porous, anisotropic film for biomimicking of endothelial basement membrane. ACS Appl Mater Interfaces. doi:10.1021/acsami.5b02464.
- Catalog Number
STA-802-CB - Supplier
Cell Biolabs - Size
- Shipping
Dry Ice
Price
568,00 €
