Rac1/Cdc42 Activation Assay Combo Kit
| Specifications | |
|---|---|
| Product Category: | Small GTPase Activation Assays |
Product Description
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rac and Cdc42, 21 kDa proteins, belong to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene transcription, and cell transformation.
Like other small GTPases, Rac and Cdc42 regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac or Cdc42 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.
Cell Biolabs' Rac/Cdc42 Activation Assay utilizes PAK PBD Agarose beads to selectively isolate and pull-down the active form of Rac or Cdc42 from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac or Cdc42 is detected by western blot analysis using an anti-Rac or Cdc42 antibody.
Cell Biolabs Rac/Cdc42 Activation Assay Kit provides a simple and fast tool to monitor the activation of Rac or Cdc42. The kit includes easily identifiable PAK1 PBD Agarose beads, pink in color, and a GTPase Immunoblot Positive Control for quick Rac or Cdc42 identification. Each kit provides sufficient quantities to perform 20 assays for each small GTPase.
Product Citations
- Schiapparelli, P. et al. (2017) NKCC1 regulates migration ability of glioblastoma cells by modulation of actin dynamics and interacting with cofilin. EBioMedicine doi:10.1016/j.ebiom.2017.06.020.
- Galic, M. et al. (2014) Dynamic Recruitment of the Curvature-Sensitive Protein ArhGAP44 to Nanoscale Membrane Deformations Limits Exploratory Filopodia Initiation in Neurons. Elife. doi: 10.7554/eLife.03116.
- Holmes,K.M.et al. (2012) nsulin-Like Growth Factor-Binding Protein 2-Driven Glioma Progression is Prevented by Blocking a Clinically Significant Integrin, Integrin-Linked Kinase, and NF-B Network. Proc Natl Acad Sci 109:2168-2173
- Chen, H. et al. (2010) Integrity of SOS1/EPS8/ABI1 Tri-Complex Determines Ovarian Cancer Metastasis. Cancer Res. 70:9979-9990.
- Sultana, H. et al. (2010) Anaplasma phagocytophilum induces actin phosphorylation to selectively regulate gene transcription in Ixodes scapularis ticks. J Exp.
- Catalog Number
STA-404-CB - Supplier
Cell Biolabs - Size
- Shipping
Blue Ice
