OxiSelect™ 96-Well Comet Assay Kit
| Specifications | |
|---|---|
| Research Area: | Oxidative Stress |
| Assay Category: | DNA/RNA Damage and Repair Assays |
| Detection Method: | Fluorescence Microscopy |
Product Description
- Useful screening tool for various types of DNA damage
- Slides are specially treated for adhesion of low-melting agarose
- Easy visualization by epifluorescence microscopy
Assay Principle
The OxiSelect 96-Well Comet Assay is a single cell gel electrophoresis assay (SCGE) for simple evaluation of cellular DNA damage. First, individual cells are mixed with molten agarose before application to the OxiSelect 96-Well Comet Slide. These embedded cells are then treated with a lysis buffer and alkaline solution, which relaxes and denatures the DNA. Finally, the samples are electrophoresed in a horizontal chamber to separate intact DNA from damaged fragments. Following electrophoresis, the samples are dried, stained with a DNA dye, and visualized by epifluorescence microscopy. Under these conditions, the damaged DNA (containing cleavage and strand breaks) will migrate further than intact DNA and produce a “comet tail" shape. Each kit provides sufficient reagents to perform up to 96 assays.
Product Citations
- Coric, V. M. et al. (2016) Combined GSTM1-Null, GSTT1-Active, GSTA1 Low-Activity and GSTP1-Variant Genotype is associated with increased risk of clear cell renal cell carcinoma. PLoS One.
- Aydın, E. et al. (2014) The effect of carvacrol on healthy neurons and N2a cancer cells: some biochemical, anticancerogenicity and genotoxicity studies. Cytotechnology.
- Jones, D. A. et al. (2014) Changes in markers of oxidative stress and DNA damage in human visceral adipose tissue from subjects with obesity and type 2 diabetes. Diabetes Res Clin Pract.
- Tyagi, A. et al. (2011) Resveratrol Selectively Induces DNA Damage, Independent of Smad4 Expression, in its Efficacy Against Human Head and Neck Squamous Cell Carcinoma. Clin.
- Catalog Number
STA-355-5-CB - Supplier
Cell Biolabs - Size
- Shipping
RT
