OxiSelect™ Oxidative RNA Damage ELISA Kit (8-OHG Quantitation) (96 tests)

Product Description

The OxiSelect Oxidative RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHG in urine, serum, cerebrospinal fluid or other cell or tissue RNA samples. The quantity of 8-OHG in unknown sample is determined by comparing its absorbance with that of a known 8-OHG standard curve. The kit has an 8-OHG detection sensitivity range of 300 pg/ml to 40 ng/ml. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

Assay Principle

The Oxidative RNA Damage ELISA kit is a competitive ELISA for the quantitative measurement of 8-OHG. The unknown 8-OHG samples or 8-OHG standards are first added to an 8-OHG/BSA conjugate preabsorbed EIA plate. After a brief incubation, an anti-8-OHG monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 8-OHG content in unknown samples is determined by comparison with predetermined 8-OHG standard curve.