OxiSelect™ Oxidative DNA Damage Quantitation Kit (AP sites)

Product Description

Oxidative damage to DNA is a result of the interaction of DNA with reactive oxygen species (ROS), in particular, the hydroxy radical which is converted from superoxide and hydrogen peroxide by the Fenton reaction.

Oxidative attack by hydroxy radical on the deoxyribose moiety will lead to the release of free bases from DNA, generating strand breaks with various sugar modifications and simple abasic sites (AP sites).

In fact, AP sites are one of the major types of damage generated by ROS. It has been estimated that endogeneous ROS can result in about 2 x 10⁵ base lesions per cell per day.

ARP (Aldehyde Reactive Probe) reagent reacts specifically with an aldehyde group which is the open ring form of the AP sites. The reaction makes it possible to detect DNA modifications that result in the formation of an aldehyde group.

After treating DNA containing AP sites with ARP reagents, AP sites are tagged with biotin residues, which can be quantified using avidin-biotin assay followed by a colorimetric detection.

The DNA Damage Quantification Kits contain the necessary reagents for convenient determination of abasic sites in sample DNA in 96-well plate format.