OxiSelect™ UV-Induced DNA Damage ELISA Combo Kit (CPD / 6-4PP Quantitation)

Product Description

The OxiSelect Oxidative UV-induced DNA Damage ELISA Combo Kit (CPD / 6-4PP Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of CPD or 6-4PP in any DNA samples. The quantity of CPD or 6-4PP in unknown sample is determined by comparing its absorbance respectively with that of a known CPD-DNA or 6-4PP-DNA standard curve. CPD and 6-4PP are assayed individually in separate wells, and each kit provides sufficient reagents to perform up to a total of 96 assays for CPD and 6-4PP combined, including standard curve and unknown samples. The 96 wells may be divided between CPD and 6-4PP assays at any ratio (e.g. 64 wells for CPD and 32 wells for 6-4PP, or 48 wells for each target).

Assay Principle

CPD or 6-4PP-DNA standards or unknown DNA samples are first heat denatured before adsorbed onto a 96-well DNA high-binding plate. The CPD or 6-4PPs present in the sample or standard are probed with an anti-CPD or anti-6-4PP antibody, followed by an HRP conjugated secondary antibody. The CPD or 6-4PP content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined CPD-DNA or 6-4PP-DNA standards.