Black Hole Scorpions Unimolecular Probe: 5' FAM (Emission 520 nm) Internal BHQ-1/HEG (Black Hole Quencher)

Product Description

BHQ™ (Black Hole Quencher™)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end.

Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Similar to Molecular Beacons, these primers do not require enzymatic cleavage of the probe during PCR cycling.  These qualities make Scorpions Primers valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, and gene quantification.

The BHQ™-labeled Scorpions primers incorporate two distinct structures:
1) a target-specific DNA probing sequence
2) a target-specific PCR Primer.  

Fluorophore and BHQ dye selection chart

This chart is intended to guide you through the dye selection process for your oligonucleotide. A fluorophore and quencher combination may be selected for applications such as probe-based qPCR and SNP genotyping.