COVID-19 Rapid Isothermal PCR Kit Add to Cart

Cat#: RT-LAMP-B-25-RB
Quantity: 25 tests
Price: 360 €
Supplier: RayBiotech
Shipping: Dry Ice
User Manual  

• Detects as few as 25 copies/µL of SARS-CoV-2 virus
• Results in 30 minutes (with extracted RNA)
• Only a heat block is required
• Results visual by eye
• Reagents to reduce DNA carry-over contamination included
• pH-independent


This kit from RayBiotech utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 570 nm and 650 nm.

The kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.

Image
Fig.1. Absence of viral RNA is represented by a purple color (570 nm)(see picture above). The presence of viral RNA is represented by a blue color (650 nm). An OD ratio of 650 nm / 570 more than 1.0 indicates that SARS-CoV-2 viral RNA is present.



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