SensiMix SYBR No-ROX Kit

Outstanding assay reproducibility and sensitivity for both DNA and cDNA templates, under standard conditions.

• Sensitive - reproducible detection of low-copy number templates
• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
• Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types

Product Description

The SensiMix™ SYBR® No-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that do not require a passive reference dye for normalization of well-to-well differences.

A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® No-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.

The SensiMix SYBR® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

• Gene expression analysis
• DNA / cDNA target detection
• miRNA profiling / quantification
• Copy number variation (CNV) analysis

Broad dynamic range SensiMix SYBR No-ROX Kit

Amplification curves were obtained for the GAPDH gene over 10 log dilution series. Reactions were in triplicate, using standard reaction conditions. The results demonstrate that the kit offers outstanding reproducibility and discrimination even across samples of low copy number templates.