PiranhaTM HEK293T GFP/Puromycin Stable Cell Line (>1x10^6 cell/vial)

Product Description

Get targeted protein degradation screens and similar studies up and running quickly with SBI’s Piranha™ Targeted Protein Degradation System, HEK293T GFP/Puromycin Stable Cell Line. Just electroporate an antibody specific for your protein-of-interest into Piranha HEK293T cells and you can start seeing degradation of the protein-of-interest in as little as 1 hour*.

SBI’s Piranha Targeted Protein Degradation System is based on the TRIM21 pathway found in most mammalian cells. Like TRIM21, the Piranha protein binds to conserved regions of an antibody, targeting the antibody—and the bound antigen—for degradation by the proteasome.

To use the Piranha HEK293T GFP/Puromycin Stable Cell Line for targeted protein degradation, simply electroporate a validated antibody specific for the protein-of-interest into target cells, wait an appropriate amount of time, and then observe the phenotypic outcome.

Because the rate of protein degradation is based upon many factors, we recommend performing initial experiments to empirically determine the degradation rate of your target protein and the optimal time for phenotypic observation.

*Based on published data by Clift et al., 2014

Reference

1. Clift D, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017 Dec 14;171(7):1692-1706.e18. doi: 10.1016/j.cell.2017.10.033.