pADL-8 Phagemid Vector

Product Description

pADL™-8 is a phagemid vector designed for the display of 8+8 type on the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence followed by a full-length copy of the gene VIII sequence and a double-SfiI cloning site to introduce foreign polypeptides on the N-terminal side of the major coat gene VIII protein. Expression of the fusion is under the tight control of a lac promoter. 

Vector Map

Cloning Site

Applications

• Phage display at the N-terminal side of gene VIII protein of the filamentous bacteriophage.
• Polypeptide display.
• Phage display peptide libraries.

Specifications

General characteristics:

• Plasmid Size: 2857
• Promoter: lac promoter
• Leader Peptide: PelB
• Cloning Site: double-BglI/SfiI, NotI-SpeI
• Fusion Protein: full length gene VIII protein
• Selection: ampicillin
• Replication: oriF1, pMB1

Physical Characteristics:

• Concentration: 0.5 µg/ µl
• Product Size: 10 µg
• Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile)
• Storage Temperature: -20°C

Related Products

As helper phage for the preparation of virions we recommend Interference-Resistant Helper Phage CM13.