Mpox Virus (MPXV) Clade I & II PCR Nucleic Acid Detection Kit

Product Description

Mpox virus (formerly known as the 'Monkeypox virus') is a virus from the orthopox family of viruses, a family most often associated with the more severe smallpox virus (variola), or the origination of vaccinations from the cowpox virus. Originally named for an outbreak among monkeys, it was first identified in humans in central and western Africa around 1970 where it continues to circulate at a low endemic level. There are two known clades of Mpox now referred to as Clade I (former Congo Basin clade) and Clade II (former West African clade). Clade II consists of two subclades, IIa and IIb. Strains detected in the 2022 global outbreak belong to Clade IIb. Clade I leads to more severe illness and a higher rate of mortality. CDC reports show that some Clade I outbreaks have killed up to 10% of the people who get infected, while Clade II infection has less severe symptoms and more than 99.9% of people survive.

The RayBiotech Mpox Virus Clade I & II PCR Nucleic Acid Detection Kit is a ready to use PCR assay for the detection of both Clade I and Clade II Mpox virus DNA in a liquid sample. This kit uses two sets of specific primers and probes in different fluorescent channels to detect the C3L gene for Clade I and G2R gene for Clade II. Each set of primers and probes are designed to target the specific region in the genome where Clades I and II have multiple differing mutations so that the two strains can be differentiated. Primer and probe for internal control, RNase P are also integrated in the assay for quality validation.

Specifications

Sensitivity: Clade I: 15 copies/μL; Clade II: 5 copies/µL
Species Detected: Virus
Compatible Sample Types: DNA
Method of Detection: PCR
Quantitative/Semi-Quantitative: Qualitative
Protein Name: C3L, G2R

This test is for Research Use Only (RUO).

Not for diagnostic or therapeutic use.