EpiQuik One-Step DNA Hydrolysis Kit

Product Description

The EpiQuik™ One-Step DNA Hydrolysis Kit is a complete set of essential components which enables the experimenter to rapidly hydrolyze DNA to deoxynucleosides with a process that can be performed in a single incubation. In comparison to currently used methods for digesting DNA with tri-enzymes, which have several drawbacks and excessive time consumption, the EpiQuik™ One-Step DNA Hydrolysis Kit provides a highly efficient enzymatic hydrolysis in minimal time. It is also very suitable for digesting DNA from various biological materials. 

• A fast one-step procedure, which can be finished in as short as 1 hour
• Performed in a single incubation, without the need for DNA denaturation
• Highly efficient enzymatic hydrolysis: non-specifically digests DNA or oligonucleotides into single nucleosides
• 96-well microplate format makes the sample preparation flexible for use with high throughput formats

Performance Data

Fig.1. 0.5 ug and 2 ug of fully methylated HeLa DNA were treated with the kit at 37C for 1 h and then analyzed by gel electrophoresis. 1: 2 ug of undigested DNA; 2: 0.5 ug of digested DNA; 3: 2 ug of digested DNA; 4: 1 ug of deoxynucleoside; “M” is a 2 kb DNA ladder.

Fig.2. 1 ug of fully methylated HeLa DNA was digested with the kit at 37C fro 1 and 2 h, respectively. The digested DNA was fluorescently quantified. 1: Blank; 2: Deoxynucleoside control; 3: undigested DNA; 4: undigested tri-oligos (3 nt); 5: undigested penta-oligos (5 nt); 6: DNA digested for 1 h; 7: DNA digested for 2 h.