EpiQuik Total Histone Extraction Kit Add to Cart

Cat#: OP-0006-100-EP
Quantity: 100 reactions
Price: 233 €
Supplier: Epigentek
Shipping: RT
User Manual  

• Histone extraction from mammalian cells and tissues
• Fast protocol, completed within 60 minutes
• Total histone protein yield appr. 0.4 mg per 107 cells or 100 mg tissues

Histones are the chief protein components of chromatin in biology. They act as spools around which DNA winds, and they play a role in gene regulation. The core histones include H2A, H2B, H3 and H4. Histones undergo posttranslational modifications which alter their interaction with DNA and nuclear proteins.
H3 and H4 histones have long tails protruding from the nucleosome which can be covalently modified at several places. Modifications of the tail include methylation, acetylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP-ribosylation. H2A can also be modified. Combinations of modifications are thought to constitute a code, the so-called "histone code." Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis).

The EpiQuik Total Histone Extraction Kit provides a simple and selective method for extracting histone proteins from mammalian cells and tissues, Resulting histones can be used for a variety of applications which include histone modification such as acetylation, methylation and sumoylation. The EpiQuik Total Histone Extraction Kit is specifically designed to meet the requirements of histone extracts used in EpiQuik assays. After treatment with pre-lysis, lysis and balance buffers, the total histones are easily extracted for immediate use or storage at proper conditions. The protocol is very fast, histone extraction can be finished within 60 minutes. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg tissues. The yield may vary depending on cell or tissue type.


Total Histone Extraction Procedure

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Quantification of acetylated Histone H3-K9

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Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit, and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3-K9 Quantification Kit (Fluorometric; Cat#P-4011-48-EP).

Related Links

Acetylated Histone Detection
Methylated Histone Detection
Histone Deacetylase and Acetyltransferase Assays
Histone Antibodies


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