Glutathione Peroxidase (GPX) Activity Assay Kit (Colorimetric)

Product Description

Glutathione peroxidase (GPX) is a tetramer composed of four identical subunits. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione peroxidase belongs to an enzyme family whose function is to detoxify peroxides in the cell. Because peroxides can decompose to form highly reactive radicals, the GPX enzymes play a critical role in protecting the cell from free radical damage, particularly lipid peroxidation.

Glutathione Peroxidase (GPX) Activity Assay Kit offers a user-friendly, reliable, and highly sensitive method for quantifying GPX activity in plasma, serum, cell lysates, and other biological fluid samples. In this assay, GPX reduces hydroperoxides, producing oxidized glutathione (GSSG), which is then recycled back to its reduced state by glutathione reductase (GR) and NADPH. The reduction of GSSG to GSH is accompanied by the oxidation of NADPH to NADP+, leading to a decrease in absorbance at 340nm. The rate of decrease in the absorbance is directly proportional to the GPX activity in the sample.