Beta-hydroxybutyrate Assay Kit

To quantify β-hydroxybutyrate in biological samples as low as 10 μM.

β-hydroxybutyrate (BHB) is a ketone body produced during periods of prolonged fasting, low carbohydrate intake, or intense exercise. BHB serves as an alternative energy source for various tissues, including brain, heart, and skeletal muscle, when glucose availability is limited. BHB also acts as a signaling molecule, affecting gene expression, oxidative stress, inflammation and other cellular processes. AkrivisBio’s β-hydroxybutyrate assay is a simple, sensitive method of determining BHB content in samples as low as 10 μM. In the assay, BHB is oxidized to acetoacetate forming NADH, which then reduces a nearly colorless tetrazolium to a highly colored formazan measured at OD 450 nm. The measurement of β-hydroxybutyrate levels is often used to assess ketosis, a metabolic state characterized by increased production and utilization of ketone bodies.