Lipolysis Assay Kit, free fatty acids detection (5 plates, reagents + buffers only)

Product Description

This lipolysis assay kit provides the tool to study chemical compounds that may influence lipolysis in cultured human adipocytes.

Principle of the Assay
Assessment of lipolytic activity is through a coupled reaction to measure nonEsterified fatty acids (NEFA) released by adipocytes. The initial step, carried out by acyl-CoA synthetase (ACS), produces fatty acyl-CoA thiol esters from the NEFA, ATP, Mg, and CoA in the reaction. The acyl-CoA derivatives react with oxygen in the presence of acyl-CoA oxidase (ACOD) to produce hydrogen peroxide. Hydrogen peroxide in the presence of peroxidase (POD) allows the oxidative condensation of 3-methyl-N-ethyl-N- (beta-hydroxyethyl)-aniline with 4- aminoantipyrine which forms a purple product that absorbs light at 550nm. This allows the concentration of NEFA to be determined from the optical density measured at 540 - 550nm.