FakirSlide - Nanostructured glass coverslips for cell culture & isolated membranes (Nanodomes- Small)

Induce diverse, well-controlled topological cues on live cells or planar model membranes

Product Description

FakirSlide are ready-to-use coverslips patterned with micro- and nano-structures to induce diverse, well-controlled topological cues on live cells or planar model membranes. Made of borosilicate glass, they are compatible with all types of advanced microscopies and provide a solid support allowing for long-term experiments.  

Kit Content

• Ready-to-use FakirSlide structured coverslip(s)
  
  (5 other nanostructures are available, see Shape Catalog below)
• 1 Stencell (Solo, Quartett or Nonet) per coverslip for optimized coating & multiplied experimental conditions
  
  

Unlock new insights! Based on a newly-developed nanostructur​ation technique called soft nanoimprint lithography, Fak​irSlide pushes the ​resolution boundarie​s and makes availabl​e to researchers a d​iverse panel of reproducible micro to nano​-scale topographies ​of various aspect ratios to better investigate key cellular responses to specific membrane topologies.

Maximize your assay reliability - FakirSlide provides ordered arrays of structures with high control over their shape, diameter and periodicity. They were specifically designed to induce robust & consistent membrane curvatures and facilitate data analysis.

Simplify your experimental workflows: made of a high-quality synthetic silica layer, the Fakirslide surface is directly functional for supported lipid bilayers without the need for harsh cleaning or hydroxylating pre-treatments. ​

FakirSlides substrates have been successfully used to manipulate membrane morphology of living cells and supported lipid bilayers, and observe effects of curvatures on membrane protein dynamics, cytoskeletal reorganization and cell migration. Check out relevant publications and example results in our dedicated sections.

Applications

Use FakirSlide to apply membrane deformations on cultured cells or membrane-mimetic systems. Experimental outputs include:

• Live cell imaging
• Immunostaining
• Migration assays 

Compatible imaging modes: confocal microscopy, Airyscan microscopy, TIRF, super-resolution microscopy (2D and 3D STED, PALM/STORM), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM).

Cell types: So far, FakirSlide has been successfully used with a variety of human (HeLa, U-2 OS, HT1080, SUM159, RPE-1, THP-1 & human monocyte-derived dendritic cells (moDC)) and murine (C2C12 mouse myoblasts) cells. 

Supported membranes: So far, FakirSlide has been successfully used with a variety of neutral (DOPC, Egg-PC, POPC, POPE & Egg-PE) and negatively-charged (Liver-PI, Brain-PS, Brain-PI(4,5)P2 & Brain-PI4P) lipid mixtures. 

Shape Catalog of FakirSlide

Specifications

Surface topographies: round or square pillars, nanodomes or nanocones
Approx. pattern area: 1 cm x 1 cm
Surface material: borosilicate glass
Coverslip diameter: 25 mm
Coverslip thickness N°: 1.5H (0.170 mm ± 0.005 mm)
Cell culture treated: No
Storage: can be stored indefinitely at room temperature when protected from dust & humidity