Recombinant Cas9 protein with NLS
| Specifications | |
|---|---|
| Source: | E. coli |
Background
Product Description
Performance Data
Figure 1. Cas9 protein cleavage of HUWE DNA fragments in vitro. Three different sgRNA targeting the HUWE gene were designed and pre-incubated with Cas9 proteins respectively for the cleavage assay. Cleaved DNA bands are indicated by red marks. Ct, uncut HUWE DNA fragment control.
Figure 2. Cas9 performance comparison. HUWE DNA fragments were used as template. The sgRNA targeting the HUWE gene was pre-incubated with Cas9 protein for the cleavage assay. The GeneHero™ Cas9 Nuclease (lane 3-5) and IDT Alt-R® Cas9 nuclease (lane 6-8) were serial diluted from 250 ng, 100 ng to 50 ng per reaction. Cleaved DNA bands are indicated by red arrows. Lane 1, assay with Cas9 only; lane 2, assay with sgRNA only.
Figure 3. Cas9 Nuclease genome modification in HEK293 cell line. Cas9 nuclease was co-transfected with synthetic sgRNA into the HEK293 cells using CRISPR-Fectin™ Transfection Reagent. Three different sgRNA targeting the HUWE gene were designed and transfected with Cas9 protein respectively. The HUWE fragments were amplified from genomic DNA and subjected to the T7 endonuclease I assay. Cleaved DNA bands are indicated by red arrows.
