MegaFi One-Step RT-PCR Kit

Simple One Step Reaction, Superior Performance

Product Description

MegaFi^TM One-Step RT-PCR is an enzyme mix of OneScript^® Hot Reverse Transcriptase and MegaFi^TM Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.

Product Features

• Enjoy simple, one-step RT-PCR reactions
• 1300X lower error rates due to our MegaFi™ Fidelity DNA Polymerase (Cat. No. G896)
• Extremely thermostable cDNA synthesis at 50-80°C due to our OneScript^® Hot Reverse Transcriptase (Cat. No. G593)
• Excellent protection against common ribonucleases due to our RNaseOFF Ribonuclease Inhibitor