Mycoplasma qPCR Detection Kit

Reliably detect as few as 10 copies of Mycoplasma DNA

Product Description

abm’s Mycoplasma qPCR Detection Kit is a powerful addition to abm's trusted Mycoplasma Detection line, offering rapid and ultra-sensitive detection of Mycoplasma and Acholeplasma directly from cell culture supernatant—no DNA extraction, sample preparation, or gel electrophoresis required.

Features

• Rapid & Comprehensive: Identify 300+ Mycoplasma species in < 2 hours
• Ultra-Sensitive: Detect as few as 10 copies
• Highly Specific: Eliminates non-specific and NTC signals
• Ready-to-Use: Includes all required reagents, including ROX dye
• ISO 13485:2016 MDSAP Certified: Suitable for quality-controlled labs
  
  

Capable of detecting as few as 10 copies with complete elimination of no template control (NTC) signals, this qPCR-based kit delivers results in under 2 hours. It’s the ideal solution for routine monitoring of cell cultures and contamination screening, supporting reliable quality control in even the most sensitive workflows. Branded as the #1 qPCR Mycoplasma Detection Kit, it includes our proprietary 2X MegaFi™ Pro qPCR MasterMix and a separate ROX Reference Dye for broad qPCR instrument compatibility.

Questions?

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Find More Details

List of Detectable Species/Strains
ROX Machine Compatibility Chart

How It Works

Why test your cells for Mycoplasma?

Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:

• proliferation
• metabolism
• gene synthesis & processing
• adhesion properties
• and more

To safeguard your research, routine monitoring is essential. abm’s Mycoplasma qPCR Detection Kit provides a fast, ultra-sensitive, and reliable solution for regular screening of your cell cultures, helping to maintain data integrity and experimental reproducibility.

Performance Data

Fig.2: Mycoplasma qPCR Detection Kit (Cat. No. G240) enables robust low-copy detection with high specificity across a tenfold serial dilution of control mycoplasma DNA (10^9 to 1 copy). A 2% agarose gel analysis revealed a distinct 270 bp band at each dilution point, with signal intensity decreasing proportionally to input copy number. No non-specific amplification or primer-dimer artifacts were detected, confirming the assay’s excellent sensitivity and specificity for low-copy detection.

Fig.3: Mycoplasma qPCR Detection Kit (Cat. No. G240) accurately detects mycoplasma contamination. qPCR amplification curves show both the control DNA (Red) and a test sample (Green) positive for mycoplasma. The No Template Control (NTC) remained flat, confirming no background amplification or contamination and demonstrating the assay's high specificity.