3-D Life PVA-PEG Hydrogel FG Kit
The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels.
Product Description
Major components of the 3-D Life PVA-PEG Hydrogel Fast Gelling Kit are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.
Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001-CS) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.
Application
3-D cell cultures, hydrogel barriers
Components
| Material | Quantity | Concentration of reactive groups |
| Mal-PVA | 170 µl | 30 mmol/L |
| PEG-Link, lyophilized | 200 µl* | 20 mmol/L* |
| 10x CB (pH 5.5) | 200 µl | n.a. |
| Water | 2 x 1500 µl | n.a |
| *Volume/concentration after reconstitution of lyophilisate. | ||
3-D Life Hydrogels can be set up with various compositions. For calculation of the volumes of components please use the Calculation Tool.
For more information about the complete 3-D Life Biomimetic Hydrogel System please refer to the 3-D Life Hydrogel User Guide.
- Catalog Number
FG80-1-CS - Supplier
Cellendes GmbH - Size
- Shipping
Dry Ice
