Human MMP-2 ELISA Kit

Specifications
Reactivity:Human
Specificity:This kit is used for quantitative detection of human MMP-2
Sample Type:Cell culture supernates, serum and plasma(heparin).
Sample Size:100 ul
Sensitivity:<10pg/ml
Detection Range:156pg/ml-10,000pg/ml
Detection Method:Colorimetric
Design Principle:Sandwich
Assay Time:3.5 hours incubations; 1 hour washing and analyzing samples
Additional Information
Target Name:MMP2
Gene Symbol:MMP2
Organism:Homo sapiens
Gene ID:4313
Accn. No.:NM_001127891

Background

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.
Price
755,00 €
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