Human MMP-2 ELISA Kit
Specifications | |
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Reactivity: | Human |
Specificity: | This kit is used for quantitative detection of human MMP-2 |
Sample Type: | Cell culture supernates, serum and plasma(heparin). |
Sample Size: | 100 ul |
Sensitivity: | <10pg/ml |
Detection Range: | 156pg/ml-10,000pg/ml |
Detection Method: | Colorimetric |
Design Principle: | Sandwich |
Assay Time: | 3.5 hours incubations; 1 hour washing and analyzing samples |
Additional Information | |
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Target Name: | MMP2 |
Gene Symbol: | MMP2 |
Organism: | Homo sapiens |
Gene ID: | 4313 |
Accn. No.: | NM_001127891 |
Background
Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability. The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization. The standard product used in this kit is recombinant human MMP-2, consisting of 631 amino acids with the molecular mass of 71KDa. The detected MMP-2 includes zymogen and active enzyme.
- Catalog Number
EA100318-OR - Supplier
OriGene - Size
- Shipping
Blue Ice
Price
755,00 €