DCFDA ROS Assay Kit

Specifications
Research Area:Oxidative Stress
Assay Category:Reactive Oxygen Species (ROS) and Antioxidant Assays
Sample Type:Live cells
Detection Method:Fluorescence plate reader

Product Description

The DCFDA ROS Assay kit utilizes 2’,7’ –dichlorofluorescin diacetate (DCFDA), a cell permeable reagent, to detect reactive oxygen species in live cells. DCFDA is capable of measuring hydroxyl, peroxyl, and other reactive oxygen species activity within the cell. When DCFDA enters the cell, it is deacetylated by cellular esterases into a non-fluorescent compound. Then, it is oxidized by reactive oxygen species into 2’, 7’ –dichlorofluorescein (DCF), which is highly fluorescent and can be detected by fluorescence spectroscopy at excitation and emission wavelengths of 485 nM and 535 nM, respectively.

For this assay, the cells are first stained with DCFDA. Then, the cells can be treated with compound(s) of interest and measured using a fluorescent plate reader.

Ea 7001 Cellular Ros

 

  • Catalog Number
    EA-7001-SO
  • Supplier
    Signosis
  • Size
  • Shipping
    Blue Ice
Price
416,00 €
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