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Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)
| Specifications | |
|---|---|
| Research Area: | Cell Metabolism |
| Assay Category: | Amino Acids and Protein Metabolism |
| Sample Type: | serum, animal tissue, urine |
| Detection Method: | Microplate reader |
| Sensitivity: | 0.04 μg/mL |
| Detection Range: | 0.04-10 μg/mL |
Product Information
The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
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