BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516

CRISPRtest Functional Cas9 Activity Assay Kit for Human Cells (for 5 cell line pairs) Add to Cart

Cat#: CRTEST-GVO-CT
Quantity: 1 kit
Price: 890 €
Supplier: Cellecta
Shipping: Dry Ice
Please note: Genetically modified organism (GVO), see General Terms.
User Manual  

Do you want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors, the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA) and the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA. Transduction of CRISPRtest virus into cells will result in a mixture of cells expressing GFP and cells expressing RFP. Upon cell growth in the presence of active Cas9, the ratio of GFP cells : RFP cells will decrease proportionally to Cas9 activity.

The CRISPRtest Functional Cas9 Activity Kit, currently for human cell lines only, is an easy-to-use, highly sensitive kit for measuring functional Cas9 activity in almost any human cell line. It is intended as a companion product for high-throughput CRISPR based genetic screening or generation of isogenic knockout cell lines.


Image
CRISPRtest workflow. Cas9 cell line and Parental cells are transduced with the CRISPRtest Virus. At Day 3 and Day 10 after transduction, a portion of the cells are analyzed by flow cytometry to determine GFP:RFP ratios. These ratios are then used to calculate the percentage knockout which is an assessment of Cas9 activity.



Image
CRISPRtest experimental data, showing flow cytometry scatter plot analysis of GFP and RFP expressing cells at Day 10 in (A) Parental and (B) Cas9-expressing HEK 293 cells. The percentages of RFP-positive cells calculated in the GFP(-) RFP(+) quadrant Q1 and GFP-positive cells in the GFP(+) RFP(-) quadrant Q3 are used to calculate the percent knockout in the table below.



Image

CRISPRtest results at Day 10. In Cas9-expressing HEK 293 cells, 98% of cells that received the gRNA targeting PCNA experienced gene knockout in both PCNA alleles.

Related Links

Lentiviral Packaging Plasmid Mix
LentiFuge Viral Concentration Reagent
CRISPR Cas9 Lentiviral gRNA Custom Constructs and Libraries
CRISPR Cas9 Lentiviral gRNA Cloning Vectors and Controls


BioCat Autumn Special

Autumn Special

Browse our list of promotions and purchase your products of interest at a very special rate.

50 Euro Voucher

Email Newsletter

Subscribe to the BioCat Email Newsletter and receive a 50 € voucher.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Support

Request a Catalog
Join our Mailing List
Contact us

Home
Imprint / Impressum
Top of Page Up!