CRISPRiTest Functional dCas9 Repressor Assay Kit for Mammalian Cells (for 5-15 cell lines)

Product Description

The Kit allows you to measure a functional dCas9-Repressor (e.g., dCas9-KRAB) in any mammalian cell system. The choice of kit (CRISPRiTest™ or CRISPRiTest™ (EF1L)), depends on the cell line to be tested. In iPS cells, where the CMV promoter used in the CRISPRiTest™ Kit is quickly silenced, the CRISPRiTest™ Kit (EF1L) is strongly recommended. 

The CRISPR interference (CRISPRi) system is a modification of the original CRISPR system that uses a targeted sgRNA molecule to direct a “catalytically dead" Cas9 (dCas9) fused with a gene repressor, for example, KRAB (i.e., dCas9-KRAB), to a target a gene promoter on genomic DNA. The recruitment of the Cas9-Repressor to the promoter region then blocks expression of the gene regulated by the promoter. The CRISPRiTest Kits provide reagents to measure the repressor efficiency of any Streptococcus pyogenes dCas9 repressor (dCas9-Rep) hybrid protein in cells designed to be used for CRISPRi experiments, using a FACS-based assay. 

The CRISPRi Test Kit contains packaged lentiviral constructs expressing dCas9-Rep regulated GFP and a non-regulated RFP protein. The Kit provides enough reagents for 5-10 cell lines.