BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516

CRISPRiTest Functional dCas9 Repressor Assay Kit for Mammalian Cells (for 5-15 cell lines) Add to Cart

Cat#: CRITEST-GVO-CT
Quantity: 1 kit
Price: 1015 €
Supplier: Cellecta
Shipping: Dry Ice
Please note: Genetically modified organism (GVO), see General Terms.
User Manual  

The CRISPRiTest Functional dCas9-Repressor Assay Kit allows you to measure a functional dCas9-Repressor (e.g., dCas9-KRAB) in any mammalian cell system. The CRISPR interference (CRISPRi) system is a modification of the original CRISPR system that uses a targeted sgRNA molecule to direct a “catalytically dead” Cas9 (dCas9) fused with a gene repressor, for example, KRAB (i.e., dCas9-KRAB), to a target a gene promoter on genomic DNA. The recruitment of the Cas9-Repressor to the promoter region then blocks expression of the gene regulated by the promoter.
The CRISPRiTest Kit provides reagents to measure the repressor efficiency of any Streptococcus pyogenes dCas9 repressor (dCas9-Rep) hybrid protein in cells designed to be used for CRISPRi experiments, using a FACS-based assay.
The Kit contains packaged lentiviral constructs expressing dCas9-Rep regulated GFP and a non-regulated RFP protein. The Kit provides enough reagents for 5-10 cell lines.


DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

BioCat Spring Special

Spring Special

10% OFF
3'UTR microRNA Target & Promoter Analysis

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!