CRISPRaTest Functional dCas9 Activator Assay Kit for Mammalian Cells (for 5-15 cell lines) Add to Cart
|Please note:||Genetically modified organism (GVO), see General Terms.|
The CRISPRaTest Functional dCas9-Activator Assay Kit measures functional dCas9 transactivator (e.g., dCas-VPH) in any mammalian cell system. The CRISPR gene activation system (CRISPRa), a modification of the original CRISPR knockout system, uses an sgRNA molecule to direct an inactive Cas9 (dCas9) fused with a gene activator, for example VP64 (i.e., dCas9-VP64), to a target location on genomic DNA near a gene promoter. The sgRNA-dCas9-Activator complex then increases expression levels of the gene regulated by the promoter.
The CRISPRaTest Kit provides reagents to measure the activator strength of any Streptococcus pyogenes dCas9 transactivator (dCas9-TA) hybrid protein in cells designed to be used for CRISPRa experiments using a FACS-based assay.
The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene that increases in expression when expressed in cells with active dCas9-TA. The kit provides enough reagents to measure this activity in 5-15 cell lines.