CytoSelect™ 24-well Cell Migration and Invasion Assay (8 µm), Colorimetric, Combo Kit

Product Description

• Fully quantify chemotaxis and cell invasion with no manual cell counting
• Includes two plates with 8 µm membrane inserts:
  - one uncoated for chemotaxis
  - one precoated on top of the membrane with ECM matrix (basement membrane) for cell invasion

If you are assaying both invasive and migratory properties of your cells, order one of our economic CytoSelect™ Cell Migration  / Invasion Assay Combo kits. These kits save you money compared to buying separate chemotaxis and cell invasion kits.

This CytoSelect 24-well Cell Migration and Invasion Combo Kit for colorimetric quantitation provides sufficient reagents to perform 12 cell migration plus 12 cell invasion assays. A combo kit for fluorometric quantitation is also available, as well as a 96-well Combo Kit.

Supporting Data

Fig.1. Migration of Human Fibrosarcoma HT-1080 Cells. Cells were seeded at 30,000 cells per well of a 24-well plate and allowed to migrate toward 10% FBS for 4 hours in either the presence or absence of 2µM Cytochalasin D. Migratory cells on the bottom of the polycarbonate membrane were stained (top) and quantified in a fluorescence plate reader (bottom).

Fig.2. Effects of Cytochalasin D on Invading Cells using the CytoSelect™ 24-Well Cell Invasion Assay. HT-1080 and NIH3T3 cells (negative control) were seeded at 300,000 cells/well and allowed to invade toward 10% FBS for 24 hrs in the presence or absence of 2µM Cytochalasin D. Invasive cells, on the bottom of the invasion membrane, were stained (above) and then quantified at OD 560 nm after extraction using a standard plate reader (not shown).