CytoSelect™ 48-well Cell Adhesion Assay (ECM Array, Colorimetric)

Product Description

• Full quantitation of cell adhesion with no manual cell counting
• Plates precoated with a uniform substrate layer of a single ECM protein in each row:
  Collagen I, Collagen IV, Fibrinogen, Fibronectin, and Laminin

Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival.

Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:

1. Cells are seeded onto the substrate
2. Adherent cells attach, while non-adherent cells are washed away
3. Adherent cells are quantified on a standard plate reader

Fig.1. CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array. Serum starved cells from three different cell lines were allowed to attach to the ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were stained according to the assay protocol. BSA = Bovine Serum Albumin. VN = Vitronectin. LN = Laminin. FN = Fibronectin. Col = Collagen.