SensiFAST SYBR & Fluorescein Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
• Sensitive – reliable quantification of low abundance targets and scarce samples
• Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
• Fast – delivers reproducible, accurate assay results in as little as 30 minutes

Product Description

The SensiFAST™ SYBR & Fluorescein Kit has been developed for fast, highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR & Fluorescein Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

• Gene expression analysis
• DNA / RNA target detection
• miRNA profiling / quantification
• Copy number variation (CNV) analysis

SensiFAST SYBR using fast cycling conditions

A fragment of γ-actin was amplified using SensiFAST SYBR & Fluorescein (red) and the results were compared with amplification using a kit from Supplier I (blue). The process using a 10 fold serial dilution of human genomic DNA over four orders of magnitude. The results illustrate that SensiFAST SYBR & Fluorescein is as faster (earlier C_t) and more efficient than Supplier I.

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