SensiFAST SYBR Hi-ROX Kit

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
• Sensitive – reliable quantification of low abundance targets and scarce samples
• Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
• Fast – delivers reproducible, accurate assay results in as little as 30 minutes

Product Description

The SensiFAST™ SYBR Hi-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX.

A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Hi-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.

The SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Applications

• Gene expression analysis
• DNA / RNA target detection
• miRNA profiling / quantification
• Copy number variation (CNV) analysis

SensiFAST SYBR using fast cycling conditions

A fragment of ubiquitin gene was amplified using SensiFAST SYBR Hi-ROX (blue) and the results were compared with amplifications using a Kit from Supplier Q (red). The process used a 10 fold serial dilution of human cDNA (in quadruplicate) over four orders of magnitude. The results illustrate SensiFAST SYBR Hi-ROX was faster (earlier C_t), with higher specificity and reproducibility.

SensiFAST Products Customer Review

SensiFAST SYBR No-ROX One-Step Kit Customer Review