1 kb HyperLadder

Ready-to-use molecular weight marker with higher intensity bands for easy orientation, used for size determination of nucleic acids from 200 bp to 10 kb.

Product Description

HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels.

The 14 regularly spaced, easy to remember bands, ranging from 200 bp to 10,037 bp, with higher intensity reference bands at 1000 bp and 10,037 bp and a doublet band at 1500/1517 bp allow for easy identification and orientation of your band at a glance.

The ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 1kb from the vial to the gel. The approximate mass of DNA in each of the HyperLadder 1kb bands is provided for optionally determining the approximate mass of DNA in comparably intense samples of similar size. The HyperLadder 1kb is also supplied with a 5x loading dye for loading of sample DNA.

With a wide size range, HyperLadder 1kb is perfect for size determination in techniques such as sequence analysis, confirmation of plasmid construction and PCR products as well as Southern blotting and other downstream techniques.

• Broad size range – 14 evenly distributed bands from 200 bp to 10 kb
• Easy to orientate – several brighter reference bands
• Stable – can be stored at room temperature for up to 6 months
• Supplied with loading dye – for loading sample DNA

Ready-to-use molecular weight marker for easy orientation and size determination of DNA up to 10 kb
5 uL HyperLadder 1kb run on a 1% TBE-agarose gel and visualized using ethidium bromide

Applications

• Restriction fragment mapping
• Sequence analysis
• Confirmation of plasmid construction
• Detection of DNA polymorphisms
• Southern/Northern blotting
• Cloning