MangoMix, 2x

MangoMix™ is a convenient, ready-to-go, 2x Reaction Mix containing MangoTaq™ DNA Polymerase, MgCl₂ and ultra-pure dNTPs manufactured by Bioline. The Mix is optimized and ready-to-use and requires only the addition of water, template and primers.

Product Description

• Direct gel loading - no need for further post-PCR processing steps
• Easy visual recognition- reduces pipetting errors
• High performance - pre-optimized 2x solutions
• Ready to use format - reduces risk of contamination and decreases the reaction set-up time
• Reproducible results - consistent QC ensures reliability

MangoMix™ is optimized and ready-to-use, so the user need only add water, template and primers. MangoMix reduces the time required to set up reactions, thereby minimizing contamination risks and providing greater reproducibility through a reduction in the number of pipetting steps. MangoMix can be loaded directly onto an agarose gel for analysis, without the need for a separate gel-loading buffer.

The presence of dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist. MangoMix has been optimized for a wide variety of templates. An additional 50 mM of MgCl₂ solution is included should any fine adjustments be required.

Applications

• High throughput applications
• Suited to a wide range of PCR assays
• Products suitable for TA cloning

Amplification of a range of fragments from different human genes using MangoTaq DNA Polymerase and supplier Q Taq DNA Polymerase

The amplification products are as follows: 119 bp (43% GC) from human glucocerebrosidase gene (1), 321 bp (37% GC) from angiotensin receptor II gene (2), 635 bp (56% GC) from rhodopsin gene (3), 762 bp (33% GC) from b-globin gene (4), 1200 bp (54% GC) from a-1-antitrypsin gene (5). PCR was performed in 50 µL reaction mixtures containing 50 ng human genomic DNA and 1.5 mM MgCl₂. HyperLadder 50bp (M).