MangoTaq DNA Polymerase

MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang, resulting in PCR product suitable for effective integration into TA cloning vectors.

• Direct gel loading - no need for further post-PCR processing steps
• Easy visual recognition- reduces pipetting errors
• Robust performance - perfect for a wide range of PCR reactions
• Reproducible results - consistent QC ensures reliability

Product Description

MangoTaq™ DNA Polymerase offers high yield across a wide range of DNA concentrations. MangoTaq DNA Polymerase leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

The polymerase is supplied with two different reaction buffers for greater flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.

The two reaction buffers supplied are: 5x Colored Reaction Buffer and 5x Colorless Reaction Buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although extremely rare exceptions may exist.

Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection. The specificity and performance of MangoTaq DNA Polymerase can be further improved with the use of 3% DMSO, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

Applications

• High throughput applications
• Suited to a wide range of PCR assays
• Products suitable for TA cloning

Amplification of a 1 kb fragment from l DNA using MangoTaq DNA Polymerase and supplier Q Taq DNA Polymerases

A 1 kb l DNA fragment was amplified using MangoTaq and Taq from supplier Q. The DNA fragment was amplified from a 5-fold serial dilution of l DNA with an initial concentration of 0.5 ng (lane 1), 0.1 ng l DNA (lane 2), 0.02 ng lDNA (lane 3), 4 pg l DNA (lane 4). PCR was performed in 50 µL reaction mixtures containing 1.5 mM MgCl₂. HyperLadder 1kb (M).