beta-Galactosidase Staining Kit

Product Description

• Beta-Galactosidase Staining Kit measures the transfection efficiency of the LacZ gene
• Simple staining protocol
• Sufficient reagents for 75 assays in 35 mm culture dishes

The β-galactosidase Staining Kit provides an easy-to-use and efficient method to determine the transfection efficiency and expression of LacZ gene. β-galactosidase catalyzes the hydrolysis of X-gal, which produces a blue color in cells expressing the transfected gene. Each kit provides sufficient quantities to perform up to 75 assays in 35 mm dishes.

Performance Data

Figure 1: X-gal staining of infected HUVEC cells and chick CAM tissue. A. HUVEC cells were
infected with purified Ad-β Gal at 50 MOI (multiplicity of infection). X-gal staining was performed
after 48 hr infection period. B. Purified Ad- β Gal was injected intravenously into a 10-day old chick
embryo, after three days, X-gal staining was performed on the CAM (chick chorioallanoic membrane)
tissue.