myTags Custom Labeled Probe Pool, 13K-27K Scale

Take advantage of a significantly enhanced specificity compared to genomic-derived probes

Product Description

myTags Custom probes from Daicel Arbor Biosciences have been demonstrated to achieve highly specific targeted F(ISH) across a wide range of applications and species. No other manufacturer matches the expertise, flexibility, and multiplex options of the myTags Custom product family to meet all your oligo FISH needs.

Expert scientists are continually innovating novel improvements to design algorithms, labeling and use protocols, and application development to maximize performance across a variety of applications.

myTags probes can be easily integrated into your existing workflows to enable (F)ISH research on chromosomal material or whole cells. The labeled probes have a convenient ready-to-use format and are available in a wide range of synthesis scales and further scales on request. myTags probes are the perfect choice for any in situ hybridization experiment.

Questions?

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Features and Benefits

Affordable and scalable: A proprietary oligo synthesis platform and flexible labeling options allow production of high-quality probe pools at extremely competitive pricing for any project size.
Flexible design: Your probes can be designed from any genome reference sequence(s), and can accommodate any cytogenetics use case, such as chromosome painting or barcoding, RNA-FISH, and much more.
Broad range of probeset sizes: An optimized design and synthesis technologies can accommodate any size target, from a single locus to a full chromosome.
Access to expertise: A team of expert scientists provide complimentary probe and project design assistance.
Easy to use: With ready-to-use labeled format and multiple optimized protocols available, myTags probes are ideal for new or expert cytogenetics ISH users.

Applications

myTags (F)ISH probes have been used successfully across a diverse range of cytogenetic applications including 3D DNA-FISH, Cryo-FISH, RNA-FISH, Oligopaints, and chromosome painting for a variety of cell types.

Identify chromosomes with multicolor FISH or barcoded signals
Assemble scaffolds into chromosomes with visual confirmation of their position
Track changes in chromosome structure–translocations, duplications, deletions, etc.
Gene mapping and gene expression
Flow cytometry
Fluorescent reporters
And more

View the protocol for your application!

Performance Data

Mytags Custom Probes Performance Pic1

Figure 1: myTags probes demonstrate higher specificity compared to genomic-derived probes. Comparing myTags synthetic probes (a) versus BAC-derived probes (b) for the human TP53 locus [ATTO 550] demonstrates that the myTags probes only hybridize to the intended locus, while the BAC-derived probes also display multiple off-target hybridizations, denoted by asterisks in (b). Both panels include myTags human chromosome 17 centromere probes [ATTO 647N] and chromosomes [stained with Hoescht 33342] pseudo-colored gray.

Mytags Custom Probes Performance Pic2

Figure 2: myTags probes enable super-resolution mapping of chromosomal spatial structures. Image credit: V. Schubert.) Oligo-FISH combined with spatial super-resolution structured illumination microscopy (3D-SIM) is a useful approach for resolving helical versus non-helical arrangement of chromatin fibers. Randall et al. (2022) used this approach to confirm that the barley metaphase chromatids are formed by a helically wound ~400 nm chromatin fiber, termed chromonema.
Additionally, by measuring the volume of oligo-FISH painted regions, they were able to calculate levels of chromatin compaction. For more information, visit the original publication: Randall et al. (2022). Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes. Nucleus 13(1): 279-301.

Visit Daicel Arbors myTags Featured Applications Gallery to find more examples of myTags Custom probes in action.