Recombinant Human Paraneoplastic Antigen Ma1 Protein, Transfected HEK293 Cell Lysate
| Specifications | |
|---|---|
| Molecular Weight: | Recombinant protein product has a calculated molecular mass of 40 kDa. |
| Tag: | His |
| Source: | Human embryonic kidney 293 (HEK293) cells |
| Purity: | Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Format: | Lyophilized powder |
| Additional Information | |
|---|---|
| Synonyms: | Paraneoplastic Ma Antigen 1, Neuron- and Testis-Specific Protein 1, Paraneoplastic Antigen MA1, 37 KDa Neuronal Protein, MA1, Paraneoplastic Neuronal Antigen MA1. |
| Organism: | Human |
| Accn. No.: | Q8ND90 |
- Catalog Number
230-10300-100-RB - Supplier
RayBiotech - Size
- Shipping
Blue Ice
Price
271,00 €
