SNAP-ChIP K-AcylStat Panel
Product Description
A panel of distinctly modified mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4, accession numbers: H2A-P04908, H2B-O60814, H3.1-P68431 or H3.2-Q71DI3*, H4-P62805) wrapped by 147 base pairs of barcoded Widom 601 positioning sequence DNA. The mononucleosomes constitute a pool of 1 unmodified plus 22 histone H2A, H3, or H4 post-translational modifications (PTMs, created by a proprietary semi-synthetic method): H3K4ac, H3K9ac, H3K14ac, H3K18ac, H3K23ac, H3K27ac, H3K36ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac, H4K20ac, H3K9bu, H3K9cr, H3K18bu, H3K18cr, H3K27bu, H3K27cr, H3K27acS28phos, H3K4,9,14,18ac, H4K5,8,12,16ac, H2AK5,8,13,15ac. Each distinctly modified nucleosome is distinguishable by a unique sequence of DNA ( barcode‚) at the 3' end that can be deciphered by qPCR or next-generation sequencing. Each of the 23 nucleosomes in the pool is wrapped by 2 distinct DNA species, each containing a distinct barcode (‚A‚ and B‚, see SNAP-ChIP Manual) allowing for an internal technical replicate. * Histone H3.2 contains a Cys to Ala substitution at position 110.
Product Citations
- Dimitris Karagiannis, Warren Wu, Albert Li, Makiko Hayashi, Xiao Chen, Michaela Yip, Vaibhav Mangipudy, Xinjing Xu, Francisco J. Sánchez-Rivera, Yadira M. Soto-Feliciano, Jiangbin Ye, Thales Papagiannakopoulos, Chao Lu (2024) Metabolic reprogramming by histone deacetylase inhibition preferentially targets NRF2-activated tumors Cell reports
- Maya M Arce, Jennifer M Umhoefer, Nadia Arang, Sivakanthan Kasinathan, Jacob W Freimer, Zachary Steinhart, Haolin Shen, Minh T N Pham, Mineto Ota, Anika Wadhera, Rama Dajani, Dmytro Dorovskyi, Yan Yi Chen, Qi Liu, Yuan Zhou, Danielle L Swaney, Kirsten Obernier, Brian R Shy, Julia Carnevale, Ansuman T Satpathy, Nevan J Krogan, Jonathan K Pritchard, Alexander Marson (2024) Central control of dynamic gene circuits governs T cell rest and activation. Nature
- Karagiannis Dimitris, Wu Warren, Li Albert, Hayashi Makiko, Chen Xiao, Yip Michaela, Mangipudy Vaibhav, Xu Xinjing, Sánchez-Rivera Francisco J., Soto-Feliciano Yadira M., Ye Jiangbin, Papagiannakopoulos Thales, Lu Chao (2023) Metabolic Reprogramming by Histone Deacetylase Inhibition Selectively Targets NRF2-activated tumors bioRxiv
- Yang Haopeng, Zhang Wenchao, Ravanmehr Vida, Mechaly Ariel, Regad Leslie, Haouz Ahmed, Chen Ruidong, Henderson Jared M., Rojas Estela, Wilson Ashley, Parsons Sydney, Nastoupil Loretta, Davis R. Eric, Deng Qing, Samaniego Felipe, Rodrigues-Lima Fernando, Green Michael R. (2023) CREBBP lysine acetyltransferase domain mutations create zombie enzymes that alter chromatin loading dynamics and prevent EP300 redundancy bioRxiv
- Aliaksandra Radzisheuskaya, Pavel V Shliaha, Vasily V Grinev, Daria Shlyueva, Helene Damhofer, Richard Koche, Vladimir Gorshkov, Sergey Kovalchuk, Yingqian Zhan, Keli L Rodriguez, Andrea L Johnstone, Michael-C Keogh, Ronald C Hendrickson, Ole N Jensen, Kristian Helin (2021) Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis. Molecular cell
- Catalog Number
19-3001-EPC - Supplier
EpiCypher - Size
- Shipping
Blue Ice
Price
466,00 €
