CUTANA CUT&Tag Kit Version 3, Primer Set 2

Ultra Low Input Chromatin Mapping Assay

Product Description

• Fast: Cells to sequencing in 2 days
• Streamlined: Exclusive single-tube protocol, no library prep
• High Sensitivity: Reliable data down to 10,000 cells
• Scalable: Designed for multi-channel pipetting and 8-strip tubes
• Dramatic cost savings: Only 5-8 million sequencing reads

Cleavage Under Targets and Tagmentation (CUT&Tag) uses a fusion of protein A, protein G, and Tn5 (pAG-Tn5) to selectively target antibody-labelled chromatin for high-resolution sequencing analysis. EpiCyphers CUTANA CUT&Tag Kit enables the use of this innovative mapping assay.

The CUTANA CUT&Tag Kit offers a comprehensive solution for ultra-sensitive mapping of histone post-translational modifications (PTMs). This kit uses an exclusive Direct-to-PCR strategy to go from cells to PCR-amplified sequencing libraries in one tube, bypassing traditional library prep and minimizing sample loss. The protocol is also designed for compatibility with multi-channel pipetting for increased throughput and reproducibility. 

The recommended input for CUT&Tag is 100,000 native nuclei per reaction. Comparable data can be generated down to 10,000 nuclei, and the protocol is also validated for whole cells, cryopreserved samples, and lightly cross-linked nuclei or cells. CUT&Tag provides robust profiling for histone PTMs. For chromatin-associated proteins (e.g. transcription factors), CUT&RUN is recommended.

Multiplexed Primers

The CUTANA Cut & Tag Kit is available with two different primer sets. Each kit contains combinatorial dual indices for multiplexed sequencing of up to 48 reactions. Customers can combine the kits to multiplex up to 96 reactions:

CUTANA CUT&Tag Kit Version 3, Primer Set 1  (Cat.# 14-1102-EPC)
CUTANA CUT&Tag Kit Version 3, Primer Set 2  (Cat.# 14-1103-EPC)

Included Reagents

• pAG-Tn5 for targeted chromatin tagmentation
• ConA magnetic beads for immobilizing cells/nuclei
• SNAP-CUTANA™ Spike-in Controls for assay optimization and reaction monitoring
• Positive (H3K27me3, H3K4me3) and negative (IgG)
• Anti-Rabbit Secondary Antibody 
• i5 and i7 primers, PCR Master Mix, and SPRI beads for indexing PCR and library purification
• 8-strip tubes, multiplexing primers, buffers, buffer components
  
  

Learn more about the CUT&Tag Technology

Supported Applications

• Single Cell CUT&Tag
• Spatial CUT&Tag (scroll to Table of Contents on linked page and click Spatial CUT&Tag)
• Multiomic CUT&Tag

Related Products

Individual core reagents and antibodies for CUTANA CUT&Tag Assays are also available.