myBaits Targeted Sequencing

Enhance the efficiency and cost-effectiveness of your NGS research projects for any organism and any project size

• Reduce your sequencing costs per sample while increasing the accuracy and power of your data analysis
• Biotinylated baits provide the best combination of ease, efficiency, and cost-effectiveness
• Proprietary oligo synthesis platform for flexible production of high-quality bait sets for any project size

The Kits from Daicel Arbor Biosciences provide pools of in-solution biotinylated RNA probes plus reagents for highly-efficient, scalable targeted sequencing on any NGS platform, including Illumina^®, Ion Torrent^®, PacBio^®, and Nanopore^®. Daicel Arbor's proprietary oligo synthesis technology delivers high-quality, complex baits at extremely competitive pricing. In addition, we can offer complimentary bait design and project development assistance from a team of expert scientists.

myBaits kits have been successfully used in thousands of research projects from a wide variety of genome types (e.g. animals, plants, viruses and microbes) and DNA sources (e.g. fresh, degraded, and environmental). 

Many biological sample types are dominated by DNA from other sources, requiring significant next-generation sequencing (NGS) depths to resolve specific microbial genomes or to fully characterize the variation within microbial communities. Targeted sequencing that excludes background non-target DNA from samples prior to NGS solves this problem and dramatically reduces the overall costs of sequencing and data analysis per sample. Hybridization capture is the most versatile technique for comprehensive, cost-effective targeted NGS of e.g. viruses and bacteria in complex samples.

Questions?

Connect with us today to use the advantages of myBaits targeted sequencing.

Key Benefits

• Cost savings—Focus your NGS on targets of interest, only pay for data you need
• Superior performance—Optimized chemistry and protocol for high, even coverage
• Probe design service—Includes project and panel design assistance from our scientists
• Open platform—Compatible with any NGS library preparation system
• Scalable—Different panel and kit sizes available for any project scale
• Complete solution—Convenient kits include hybridization and wash reagents

How does myBaits work?

Pair with library prep for a complete workflow

The Library Prep Kit for myBaits provides reagents to prepare NGS libraries from dsDNA samples (with user-supplied adapters) as well as for the post-capture amp step of the myBaits protocol. Paired with myBaits, the Library Prep Kit offers a seamless solution to generating high-quality targeted DNA sequencing data. 

Comprehensive Product Range

myBaits Custom Kits

Kits tailored for specific target regions are the most popular choice, allowing enrichment of specific SNPs, exons, genes, and other sequence motifs from genomic or metagenomic samples.

• Target Capture Kits for DNA- and RNA-Seq
• Target Capture Kits for Methyl-Seq
• Custom Whole Genome Enrichment (WGE) Kit

myBaits Expert Kits and other Predesigned Panels

Daicel Arbor Biosciences also offers a variety of predesigned kit options for specific research applications, such as mitochondrial DNA sequencing, ultraconserved element sequencing, whole-genome enrichment from metagenomic samples, and human cancer exome research.

• Virus - SARS-CoV-2
• 16S-Hyb - Amplicon-free targeted sequencing of the total 16S rRNA gene
• Wheat Exome - Sequence the exome of Triticeae and relatives
• Mito Panel - Predesigned bait kits for mitogenome sequencing
• Onconome - Efficiently sequence thousands of exons in hundreds of human cancer-related genes
• Human Whole Genome Enrichment (WGE) - Achieve genome-scale DNA sequencing from complex sources
• UCE (Ultraconserved Elements) - Rapidly sequence UCEs for phylogenetics research
• Plant - Angiosperms - Sequence hundreds of single-copy loci in angiosperms
• Plant - Compositae - Rapidly sequence hundreds of orthologous nuclear loci from the Daisy Family, Compositae (Asteraceae)
• Microbial Collection - Complete genome sequencing of bacterial, viral and parasite pathogens

myBaits panels as part of myReads DNA/RNA NGS Service

Looking for a complete service that includes NGS library preparation, hybridization capture with myBaits, and sequencing? We offer flexible, customizable options for different sample and project types. Daicel Arbor destilled their collective decades of NGS experience and hands-on skills from processing tens of thousands of samples in the myReads DNA/RNA Next Generation Sequencing Service.

Typical example of visualized NGS reads

Figure 2. NGS reads from enriched human gDNA library. Single-end NGS reads are shown aligned to the hg38 genomic reference sequence. Positions of the four 80nt myBaits probes in this region are indicated by blue bars. Unique read coverage is highest across the baited region, and tapers off upstream and downstream of the baited region. If sequencing further into the known or unknown flanking regions is desired, simply increase the length of your NGS library molecules (compatible with any myBaits kit).

Selected Citations

• Chyi Yin Gwee. et al. (2026) Performance of Two Custom Probe Kits for In-Solution Enrichment of Ancient Avian DNA. Molecular Ecology Resources, Vol.26, e70071. https://onlinelibrary.wiley.com/doi/full/10.1111/1755-0998.70071
• Köndgen, S. et al. (2024) A robust, scalable, and cost-efficient approach to whole genome sequencing of RSV directly from clinical samples. Journal of Clinical Microbiology 0, e01111-23. https://doi.org/10.1128/jcm.01111-23
• Matiasek, K. et al. (2023) Mystery of fatal ‘staggering disease’ unravelled: novel rustrela virus causes severe meningoencephalomyelitis in domestic cats. Nat Commun 14, 624. https://doi.org/10.1038/s41467-023-36204-w
• Ruscher, C. et al. (2023) Ecological and clinical evidence of the establishment of West Nile virus in a large urban area in Europe, Berlin, Germany, 2021 to 2022. Eurosurveillance 28, 2300258. https://doi.org/10.2807/1560-7917.ES.2023.28.48.2300258
• Ulrich, L. et al. (2022) Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not Beta. Nature 602, 307–313. https://doi.org/10.1038/s41586-021-04342-0
• Weiss, S. et al. (2022) Kiwira Virus, a Newfound Hantavirus Discovered in Free-tailed Bats (Molossidae) in East and Central Africa. Viruses 14, 2368. https://doi.org/10.3390/v14112368
• Wertheim, J.O. et al. (2021) Discovery of Novel Herpes Simplexviruses in Wild Gorillas, Bonobos, and Chimpanzees Supports Zoonotic Origin of HSV-2. Molecular Biology and Evolution 38, 2818–2830. https://doi.org/10.1093/molbev/msab072
• Wylezich, C. et al. (2021) Next-generation diagnostics: virus capture facilitates a sensitive viral diagnosis for epizootic and zoonotic pathogens including SARS-CoV-2. Microbiome 9, 51. https://doi.org/10.1186/s40168-020-00973-z
• Binder, F. et al. (2020) Isolation and characterization of new Puumala orthohantavirus strains from Germany. Virus Genes 56, 448–460. https://doi.org/10.1007/s11262-020-01755-3
• Hiltbrunner, M. and Heckel, G. (2020) Assessing Genome-Wide Diversity in European Hantaviruses through Sequence Capture from Natural Host Samples. Viruses 12, 749. https://doi.org/10.3390/v12070749
• Forth, J.H. et al. (2019) A Deep-Sequencing Workflow for the Fast and Efficient Generation of High-Quality African Swine Fever Virus Whole-Genome Sequences. Viruses 11, 846. https://doi.org/10.3390/v11090846