Total RNA-seq with complete workflow integration
Benefit from robust multiplexing with advanced barcoding
RNA Library Preparation Solutions
Efficient, reproducible RNA sequencing starts with library preparation solutions that are robust across sample types and input qualities. The Total RNA Library Kits portfolio from Revvity is designed to streamline workflows while maintaining high data quality, enabling confident transcriptome profiling in both academic and commercial research settings.
Optimized Workflows for Total RNA Sequencing
Revvity’s solutions integrate key steps of RNA library preparation into cohesive, time-efficient workflows. From strand-specific library construction to targeted ribosomal RNA removal and flexible indexing strategies, each component is engineered for consistency and scalability.
NEXTFLEX Rapid Directional RNA-Seq Kit 2.0
The NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 enables fast and reliable preparation of strand-specific RNA sequencing libraries.
- Rapid workflow: Streamlined protocol reduces hands-on time without compromising performance
- Directional library preparation: Preserves strand orientation for accurate transcript annotation
- Broad sample compatibility: Suitable for a wide range of RNA inputs and qualities
- Reproducible results: Optimized chemistry supports consistent library generation across experiments
This kit is designed for laboratories requiring high-throughput processing with dependable strand-specific data output.
Ribodepletion Solutions
Ribosomal RNA depletion is critical for maximizing informative reads in total RNA sequencing. Revvity’s ribodepletion solutions are designed to efficiently remove rRNA while preserving coding and non-coding RNA species. Two options are available: Probe based hybridization depletion with the Ribonaut rRNA Depletion Kit and CRISPR-based depletion with the Cas9-gRNA rRNA Depletion Enzyme.
- Effective rRNA removal: Enhances sequencing depth for target transcripts
- Flexible application: Compatible with diverse sample types
- Improved data quality: Reduces background signal and increases usable reads
These solutions are particularly valuable when working with degraded RNA or when poly(A) selection is not suitable.
Bead-based poly(A) Enrichment
For applications focusing on messenger RNA, Revvity’s Poly(A) Beads provide a reliable enrichment approach.
- Selective mRNA enrichment: Isolates polyadenylated transcripts with high specificity
- Consistent performance: Supports reproducible downstream library preparation
- Seamless integration: Compatible with NEXTFLEX RNA-Seq workflows
Poly(A) selection remains a preferred method for transcriptome studies requiring focused mRNA analysis.
Barcodes and Indexing Solutions
Accurate sample multiplexing is essential for efficient sequencing runs. Revvity offers UDI barcode solutions ( UDI and UDI-UMI) that integrate smoothly into RNA library preparation workflows.
- Flexible multiplexing: Enables simultaneous processing of multiple samples
- Reliable sample identification: Minimizes index misassignment
- Workflow compatibility: Designed for use with NEXTFLEX library prep kits
These indexing options support scalable study designs while maintaining data integrity.
Designed for Demanding RNA-Seq Applications
The Total RNA Library Kits portfolio from Revvity combines speed, flexibility, and reproducibility. Whether your workflow requires rapid directional library preparation, efficient ribodepletion, or precise multiplexing, these solutions are engineered to deliver consistent results across a wide range of RNA sequencing applications.
Explore the available kits and components to configure a workflow tailored to your experimental requirements.
RNA-seq Workflow
Figure 1. This figure illustrates the classic RNA-seq workflow. The numbers in the diagram show where various enrichment & depletion based accessory products can be used in RNA-seq workflows.