For immunohistochemistry applications, hapten-conjugated proteins, unlabeled proteins (OVAL, BSA, CGG and KLH) and fluorescent protein conjugates are available. In addition, we offer haptens that can be used as labeling reagents for custom conjugates.
Our line of conjugates comprises well-studied small molecule haptens, e.g. DNP, TNP, NP, NIP, and PC (Phosphoryl Choline). Various levels of conjugation density are available depending on the antibody response required. Such reagents are complete antigens, useful for inducing antibody response in animal models.
Hapten reagents (such as succinimide esters) enable you to synthesize your own custom conjugates.
GC7 is a potent inhibitor of deoxyhypusine synthase. Used as cell growth inhibitor and to synchronize the cell cycle of cultured cells.
How do I know which loading to choose for immunochemical conjugates?
In reference to immunochemicals, the word 'loading' refers to the conjugation ratio which is the average number of hapten groups conjugated to a single protein. It is generally accepted that immunization with a lower density hapten-carrier, coupled with low immunogen doses, will preferentially trigger high affinity antibodies in a lower immune response. In contrast, immunization with a high density-hapten carrier, coupled with high immunogen doses, may elicit a higher immune response as low affinity antibodies are also triggered.
Please contact us for 'loading' (conjugation ratio) information for the product of your interest.
Which conjugation ratio (loading) should I use for an ELISA?
The highest available conjugation ratio of hapten to protein (i.e. NP/BSA) should be used to capture hapten-specific antibodies in an ELISA assay.
Can I order a specific conjugation ratio for my immunochemical?
LGC Biosearch Technologies does not offer custom immunochemical productions. The conjugation ratio for any given immunochemical will vary with each production. We do not have a means of anticipating the ratio of the next production. We do not anticipate changes in performance with small modifications in molar ratio, such as 1 to 2 haptens per protein.
Are LGC Biosearch Technologies' immunochemical products alum precipitated?
Our immunochemical products are not alum precipitated and we do not offer this service. You may find a protocol for alum precipitation in the following reference:
Adjuvant effect of bacterial LPS and/or alum precipitation in response to polysaccharide and protein antigens. Seppälä IJT, Mäkelä O Immunology 1984 Dec, 53(4):827-836 PMCID: PMC1454889