CRISPR/Cas9 Gene Knockout Constructs
Genome-wide CRISPR/Cas9 Gene Knockout
Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
CRISPR Gene Knockout sgRNA vectors and viruses are highly effective at achieving knockout of your target gene. Cas9 functions to create a double-stranded break within an early exon triggering repair via Non-Homologous End-Joining (NHEJ) mechanism resulting in deleterious frameshift mutations. A comprehensive genome-wide collection of All-in-One (spCas9 and sgRNA expressing) and sgRNA only expressing constructs targeting any human, mouse, or rat gene is offered.
- Lentiviral Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
- AAV Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
- Non-Viral Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
sgRNA CRISPR/Cas9 All-in-One Lentivector Sets
Gene-specific sets of three sgRNA expression constructs designed to guide Cas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the lentivector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro.
sgRNA CRISPR/Cas9 All-in-One AAV Vector Sets
Gene-specific sets of three sgRNA expression constructs designed to guide saCas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the AAV vector pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp.
sgRNA CRISPR/Cas9 All-in-One Non-Viral Vector Sets
Gene-specific sets of three sgRNA expression constructs designed to guide Cas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the non-viral vector pNV-sgRNA-Cas9-2A-GFP.
